2022 AMI Online Salon

Polymerase Chain Reaction

Project Details

  • Entrant Name:  Hannah Barkman
  • Client: David Schumick
  • Copyright:  Hannah Barkman, 2022
  • Medium/software used: Adobe Illustrator
  • Final presentation format: Print Poster
  • Primary Audience: High School – College Age Students

Project Description

This piece is a detailed illustration of the protocol employed in Polymerase Chain Reaction (PCR). It elaborates on the components needed to complete a PCR, the processes of denaturation, annealing, polymerization, cycling and amplification, as well as the use of gel electrophoresis for quantifying levels of DNA/RNA. This piece fits on an 18″x 24″ poster format suitable for placement in a laboratory or classroom setting. The intention for this piece was to be highly informative and instructive, but also creative and engaging for any viewer. To ensure accuracy of the piece, I performed research in conducting a common PCR protocol, which helped me in understanding the steps and mechanisms of how the process works. The triadic color scheme, vector illustrations, and simple graphic design transforms complex forensic information into a cohesive, supplementary learning material for anyone interested in learning about DNA profiling and PCR protocols.