2022 AMI Online Salon

Genome-wide Genetic Perturbation Screening

Project Details

  • Entrant Name: Cassio Lynm
  • Client: New England Journal of Medicine
  • Copyright: Massachusetts Medical Society, 2022
  • Medium/software used: Adobe Illustrator, Adobe Photoshop
  • Final presentation format: Print and online journal publication
  • Primary Audience: General medical readership (physicians, healthcare professionals)

Project Description

This figure accompanies an article that discusses the clinical implications of a novel research system to identify genes of interest based on visible cell phenotypes and morphological properties. The figure depicts the researchers’ technique that utilized bar-coded Cas9 gRNA-induced gene ablation combined with a prototype high-speed image-enabled cell sorter. While the article featured a specific research focus—targeting genes that influence RelA translocation with specific vector assemblies and under specific conditions—the authors intended for readers to appreciate that the utility and efficiency of this method can have much broader application, such as cell-subpopulation analysis, guided therapeutic decision-making, and investigative drug design. Notably, each major step of their method could have been expanded into highly-detailed descriptions in their own right. Thus, in this figure, a calculated effort was made to summarize the process into three main procedural steps, to simplify the graphical representations, and to highlight unique components that have broader relevance.