2021 AMI Online Salon

SARS-CoV-2 G614 Mutant Increases ACE2 Receptor Binding

Project Details

  • Entrant Name:  Nicole Shepherd
  • Client: Isabel Romero Calvo & Evelyn Maizels
  • Copyright: Nicole Shepherd, 2021
  • Medium/software used: VMD, Pixologic ZBrush, Autodesk 3ds Max, Adobe Photoshop, Adobe Illustrator
  • Final presentation format: Digital Poster
  • Primary Audience: Biomedical professionals

Project Description

This poster tells the story about how the G614 spike protein mutant can increase ACE2 receptor binding. A schematic of the residues 614 and 859 is called out from the mutant spike protein and compared to the wild type. This depicts how the hydrogen bond in the wild type between these two residues is broken in the G614 mutant and the absence of this bond promotes the receptor binding domain(RBD) into an open conformation. Below are graphs that show the occurrence of the mutant and the wild type to be in an open or closed conformation. The mutant is more likely to have an open RBD, while the wild type is more likely to have a closed RBD. Because the mutant spike protein is more likely to be in an open state, it is able to bind to ACE2 more readily.