2020 Online Salon
Engineering Nanobodies Against Voltage Gated Sodium Channels
- Entrant Name: Sora Ji
- Membership Type: Student Submission
- Address: Baltimore, Maryland USA
- Client: Dr. Sandra Gabelli
- Copyright: 2020 Sora Ji
- Medium/software used: Adobe Photoshop CC, PyMOL
- Final presentation format: Full page illustration for a journal article, print and web
- Primary Audience: Molecular biologists
This illustration depicts the process of engineering llama-derived nanobodies against voltage-gated sodium (Nav) channels. Nanobodies are single-domain antibody fragments that can bind to an antigen.
Each step is summarized and labeled for a scientific audience. First, CT-Nav1.4 is injected into a llama. Nanobody-coding genetic material from the llama’s lymphocytes is detected with RT-PCR and inserted into E. coli for cloning. Cloned colonies are used to prepare phage display, which is screened with ELISA to determine antigen binding specificity. Nanobodies (Nb82) are purified, crystalized, and assessed for binding to an unknown site on Nav 1.4.
The steps were symbolically visualized with dynamic perspectives and vibrant colors, while maintaining colorblind accessibility. The process is organized along an organic arrow, which leads the viewer through the steps. This approach to summarizing a scientific process is intended to provide an unexpected, fresh experience for the viewer.