Graham Johnson, PhD, CMI –
The visual analysis, assembly, and communication of molecule-scale events has benefited greatly from an explosion of available tools and data that has been provided and evolved by the structural biology community over the past 40 years. Compared to molecular visualization, however, the data, tools, and techniques available for cell-scale visualization remain in a relative infancy. In structural biology, the details of a molecule can commonly be summarized in a few megabytes of data, which is heavily constrained by nanoscale physics. Could we ever uncover patterns and rules that will allow us to extract and communicate an analogously efficient understanding of cellular anatomy and behavior when faced with massive microscopy that are difficult to share and analyze? This presentation will overview the field to describe available tools and current challenges to advancement. The presentation will elaborate on approaches we have taken to annotate 3D light microscopy data with mesoscale structures modeled using information from higher-resolution information that spans biology. It will further describe whole cell and multiscale visualization tools and data that function directly in a web browser that we develop for allencell.org.
Graham Johnson is a computational biologist and Certified Medical Illustrator (CMI) with 18 years of professional experience (grahamj.com). His teams at the University of California, San Francisco (UCSF, mesoscope.org) and at the Allen Institute for Cell Science work to generate, simulate and visualize molecular models of cells. In collaboration with The Scripps Research Institute, they provide the tools ePMV and cellPACK. At AICS, Graham and his team build the Allen Cell Explorer website (allencell.org) to provide easy online access to a trove of volumetric microscopy images, analysis tools, models and scientific summaries that describe the organizational diversity of human stem cells.